法甲外围app

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法甲外围app >>> 产品展示 >>> ELISA试剂盒 >>> 小鼠elisa试剂盒  >>> 小鼠葡萄糖依赖性胰岛素释放多肽ELISA检测试剂盒
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小鼠葡萄糖依赖性胰岛素释放多肽ELISA检测试剂盒

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产品名称: 小鼠葡萄糖依赖性胰岛素释放多肽ELISA检测试剂盒
产品型号:
产品厂商: 上海心语生物科技有限公司
产品报价: 18
产品特点
我司提供小鼠葡萄糖依赖性胰岛素释放多肽ELISA检测试剂盒全部相关信息(如:价格、中英文说明书、存储、操作指南),专业供应进口分装、原装ELISA试剂盒,并拥有完善的研发系统和售后服务,熟练掌握各种酶联技术。
小鼠葡萄糖依赖性胰岛素释放多肽ELISA检测试剂盒 的详细介绍

小鼠葡萄糖依赖性胰岛素释放多肽ELISA检测试剂盒

小鼠葡萄糖依赖性胰岛素释放多肽试剂使用说明书

 

小鼠葡萄糖依赖性胰岛素释放多肽ELISA检测试剂盒 组成

 

1

30BEINONGSUOXIDIYE

20ml×1PING

7

ZHONGZHIYE

法甲外围app6ml×1PING

2

MEIBIAOSHIJI

法甲外围app6ml×1PING

8

BIAOZHUNPIN(8000pg/ml)

法甲外围app0.5ml×1PING

3

MEIBIAOBAOBEIBAN

法甲外围app12KONG×8TIAO

9

BIAOZHUNPINXISHIYE

法甲外围app1.5ml×1PING

4

YANGPINXISHIYE

6ml×1PING

10

SHUOMINGSHU

1FEN

5

XIANSEJIAYE

法甲外围app6ml×1PING

11

FENGBANMO

2ZHANG 

6

XIANSEJIBYE

6ml×1/PING

12

MIFENGDAI

1GE

 

标本要求

1.BIAOBENCAIJIHOUJINZAOJINXINGTIQU,TIQUANXIANGGUANWENXIANJINXING,TIQUHOUYINGJINKUAIJINXINGSHIYAN。RUOBUNENGMASHANGJINXINGSHIYAN,KEJIANGBIAOBENFANGYU-20℃BAOCUN,DANYINGBIMIANFANFUDONGRONG

2.BUNENGJIANCEHANNaN3DEYANGPIN,YINNaN3YIZHILAGENGUOYANGHUAWUMEIDE(HRP)HUOXING。

Materials provided with the kit(试剂盒组成

 

1

wash  solution

20ml×1bottle

7

法甲外围appStopp Solution

6ml×1 bottle

2

法甲外围appHRP-Conjugate reagent

6ml×1 bottle

8

Standard(16pg/ml)

法甲外围app0.5ml×1 bottle

3

Microelisa stripplate

12well×8strips

9

Standard diluent

法甲外围app1.5ml×1bottle

4

Sample diluent

法甲外围app6ml×1 bottle

10

法甲外围appInstruction

1

5

Chromogen Solution A

法甲外围app6ml×1 bottle

11

Closure plate membrane

2

6

Chromogen Solution B

法甲外围app6ml×1 bottle

12

法甲外围appSealed bags

1

 

Specimen requirements

1.       extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

2.       Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

小鼠葡萄糖依赖性胰岛素释放多肽试剂操作步骤

1.         BIAOZHUNPINDEXISHI:BENSHIJIHETIGONGYUANBEIBIAOZHUNPINYIZHI,YONGHUKEANZHAOXIALIETUBIAOZAIXIAOSHIGUANZHONGJINXINGXISHI。

 

4000pg/ml

5HAOBIAOZHUNPIN

150μlDEYUANBEIBIAOZHUNPINJIARU150μlBIAOZHUNPINXISHIYE

2000pg/ml

4HAOBIAOZHUNPIN

150μlDE5HAOBIAOZHUNPINJIARU150μlBIAOZHUNPINXISHIYE

1000pg/ml

3HAOBIAOZHUNPIN

150μlDE4HAOBIAOZHUNPINJIARU150μlBIAOZHUNPINXISHIYE

500pg/ml

2HAOBIAOZHUNPIN

150μlDE3HAOBIAOZHUNPINJIARU150μlBIAOZHUNPINXISHIYE

250pg/ml

1HAOBIAOZHUNPIN

150μlDE2HAOBIAOZHUNPINJIARU150μlBIAOZHUNPINXISHIYE

 

法甲外围app2.         JIAYANG:FENBIESHEKONGBAIKONG(KONGBAIDUIZHAOKONGBUJIAYANGPINJIMEIBIAOSHIJI,QIYUGEBUCAOZUOXIANGTONG)、BIAOZHUNKONG、DAICEYANGPINKONG。ZAIMEIBIAOBAOBEIBANSHANGBIAOZHUNPINZHUNQUEJIAYANG50μl,DAICEYANGPINKONGZHONGXIANJIAYANGPINXISHIYE40μl,RANHOUZAIJIADAICEYANGPIN10μl(YANGPINzuiZHONGXISHIDUWEI5BEI)。JIAYANGJIANGYANGPINJIAYUMEIBIAOBANKONGDIBU,JINLIANGBUCHUJIKONGBI,QINGQINGHUANGDONGHUNYUN。

法甲外围app3.         WENYU:YONGFENGBANMOFENGBANHOUZHI37℃WENYU30FENZHONG。  

4.         PEIYE:JIANG30BEINONGSUOXIDIYEYONGZHENGLIUSHUI30BEIXISHIHOUBEIYONG

法甲外围app5.         XIDI:XIAOXINJIEDIAOFENGBANMO,QIQUYETI,SHUAIGAN,MEIKONGJIAMANXIDIYE,JINGZHI30MIAOHOUQIQU,RUCIZHONGFU5CI,PAIGAN。

法甲外围app6.         JIAMEI:MEIKONGJIARUMEIBIAOSHIJI50μl,KONGBAIKONGCHUWAI。

7.         WENYU:CAOZUOTONG3。

8.         XIDI:CAOZUOTONG5。

法甲外围app9.         XIANSE:MEIKONGXIANJIARUXIANSEJIA50μl,ZAIJIARUXIANSEJIB50μl,QINGQINGZHENDANGHUNYUN,37℃BIGUANGXIANSE15FENZHONG.

法甲外围app10.     ZHONGZHI:MEIKONGJIAZHONGZHIYE50μl,ZHONGZHIFANYING(CISHILANSELIZHUANHUANGSE)。

11.     CEDING:YIKONGBAIKONGDIAOLING,450nmBOZHANGYIXUCELIANGGEKONGDEXIGUANGDU(ODZHI)。 CEDINGYINGZAIJIAZHONGZHIYEHOU15FENZHONGYINEIJINXING。

Assay procedure(操作步骤

1.       Dilute and add sample:Dilute Original density Standard as follow table:

 

8 pg/ml

5 Standard

150μl Original density Standard+150μl Standard diluent

4pg/ml

4 Standard

150μl 5 Standard+150μl Standard diluent

2 pg/ml

3 Standard

150μl 4 Standard+150μl Standard diluent

1 pg/ml

2 Standard

150μl 3 Standard +150μl Standard diluent

0.5 pg/ml

1 Standard

150μl 2 Standard +150μl Standard diluent

 

2.add sample:Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.

法甲外围app3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.

法甲外围app5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate:Operation with 3.

8.washing:Operation with 5.

9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
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小鼠葡萄糖依赖性胰岛素释放多肽试剂注意事项

法甲外围app1.SHIJIHECONGLENGZANGHUANJINGZHONGQUCHUYINGZAISHIWENPINGHENG15-30FENZHONGHOUFANGKESHIYONG,MEIBIAOBAOBEIBANKAIFENGHOURUWEIYONGWAN,BANTIAOYINGZHUANGRUMIFENGDAIZHONGBAOCUN。

2.NONGXIDIYEKENENGHUIYOUJIEJINGXICHU,XISHISHIKEZAISHUIYUZHONGJIAWENZHURONG,XIDISHIBUYINGXIANGJIEGUO。

法甲外围app3.GEBUJIAYANGJUNYINGSHIYONGJIAYANGQI,BINGJINGCHANGXIAODUIQIZHUNQUEXING,YIBIMIANSHIYANWUCHA。YICIJIAYANGSHIJIANzuiHAOKONGZHIZAI5FENZHONGNEI,RUBIAOBENSHULIANGDUO,TUIJIANSHIYONGPAIQIANGJIAYANG。

4. QINGMEICICEDINGDETONGSHIZUOBIAOZHUNQUXIAN,zuiHAOZUOFUKONG。RUBIAOBENZHONGDAICEWUZHIHANLIANGGUOGAO(YANGBENODZHIDAYUBIAOZHUNPINKONG*KONGDEODZHI),QINGXIANYONGYANGPINXISHIYEXISHIYIDINGBEISHU(nBEI)HOUZAICEDING,JISUANSHIQINGzuiHOUCHENGYIZONGXISHIBEISHU(×n×5)。

法甲外围app5. FENGBANMOZHIXIANYICIXINGSHIYONG,YIBIMIANJIAOCHAWURAN。

6.DIWUQINGBIGUANGBAOCUN。

7.YANGEANZHAOSHUOMINGSHUDECAOZUOJINXING,SHIYANJIEGUOPANDINGBIXUYIMEIBIAOYIDUSHUWEIZHUN.

法甲外围app8.SUOYOUYANGPIN,XIDIYEHEGEZHONGFEIQIWUDOUYINGANCHUANRANWUCHULI。

9.BENSHIJIBUTONGPIHAOZUFENBUDEHUNYONG。

10. RUYUYINGWENSHUOMINGSHUYOUYI,YIYINGWENSHUOMINGSHUWEIZHUN。

小鼠葡萄糖依赖性胰岛素释放多肽试剂保存条件及有效期

1.SHIJIHEBAOCUN:;2-8℃。

2.YOUXIAOQI:6GEYUE



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